Genetic Markers Indicate that 1,25-dihydroxyvitaminD Treatment may not Protect Against HepatocellularCarcinoma
Eurasian Journal of Medicine and Oncology, cilt.5, sa.2, ss.117-122, 2021 (ESCI, Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 5 Sayı: 2
- Basım Tarihi: 2021
- Doi Numarası: 10.14744/ejmo.2021.65874
- Dergi Adı: Eurasian Journal of Medicine and Oncology
- Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), Scopus, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.117-122
- Maltepe Üniversitesi Adresli: Evet
Özet
Objectives: The impact of 1,25-dihydroxyvitamin D on hepatocellular carcinoma (HCC) cells is a complicated area. Inthis study, we aimed to clarify the effect of 1,25-dihydroxyvitamin D on HCC cells according to genetic markers. Methods: The optimal concentration of 1,25-dihydroxyvitamin D is treated to HepG2 cells (250 nM at the 48th hour).From treated HepG2 cells, total Ribonucleic Acid (RNA) was isolated, and Ki-67, MMP-2, MMP-9, HIF-1α, hTERT, and piR 823 gene expressions were determined by SYBR Green-based real-time polymerase chain reaction. Results: : Increased expressions of Ki-67, hTERT, and piR-823 were determined compared with the control group at the48th hour after treatment (p<0.001), while decreased gene expressions of MMP-2, MMP-9, and HIF-1α were observedcompared with the control group (p<0.001). Conclusion: Currently, there are several different opinions about the usage of vitamin D, especially in HCC. In addi tion to researchers who argue that vitamin D has anticarcinogenic and protective properties, an increasing numberof researchers argue that tumor cells can become aggressive after HCC occurs. According to our results, it was deter mined that vitamin D causes HepG2 HCC cells to become aggressive in terms of gene expression in the parametersused as a marker for proliferation, adhesion, and differentiation.