Slx5/Slx8 SUMO-Targeted ubiquitin ligase deficiency shortens lifespan due to increased mutation accumulation in yeast
FEMS Microbiology Letters, cilt.372, 2025 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 372
- Basım Tarihi: 2025
- Doi Numarası: 10.1093/femsle/fnae109
- Dergi Adı: FEMS Microbiology Letters
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Artic & Antarctic Regions, BIOSIS, Chemical Abstracts Core, EMBASE, Environment Index, MEDLINE
- Anahtar Kelimeler: chronological aging, mutation accumulation, quiescence, Saccharomyces cerevisiae, Slx5/Slx8 STUbL (SUMO-Targeted ubiquitin ligase)
- Maltepe Üniversitesi Adresli: Evet
Özet
Chronological lifespan (CLS) in budding yeast Saccharomyces cerevisiae, which is defined as the time nondividing cells in saturation remain viable, has been utilized as a model to study post-mitotic aging in mammalian cells. CLS is closely related to entry into and maintenance of a quiescent state. Many rearrangements that direct the quiescent state enhance the ability of cells to endure several types of stress. Small ubiquitin-like modifier (SUMO)-Targeted ubiquitin ligases (STUbLs) play a critical role in mediating an adaptive response to various stresses. In this study, we investigated the effect of a STUbL, Slx5/Slx8, on CLS in budding yeast. We showed that both SLX5 and SLX8 deletions accelerate chronological aging, resulting in a decreased maximum and mean lifespan. slx5Δcells were capable of entering or maintaining a quiescent state during aging. On the other hand, aging slx5Δand slx8Δcells had both increased spontaneous mutation accumulation. Our data together indicate that Slx5/Slx8 STUbL is required for normal rate of aging by preventing increased spontaneous mutation accumulation during aging.