A dynamic approach reveals non-muscle myosin influences the overall smooth muscle cross-bridge cycling rate
FEBS Letters, cilt.584, sa.13, ss.2862-2866, 2010 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 584 Sayı: 13
- Basım Tarihi: 2010
- Doi Numarası: 10.1016/j.febslet.2010.04.070
- Dergi Adı: FEBS Letters
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.2862-2866
- Anahtar Kelimeler: Contractile phenotype, Force maintenance, Latch, Phasic, Tonic
- Maltepe Üniversitesi Adresli: Hayır
Özet
Recent evidence suggests that non-muscle myosin IIB (NMIIB) contributes to smooth muscle contraction. This study was designed to determine the effects of NMIIB on the cross-bridge cycling rate. The cross-bridge cycling rate was investigated using sinusoidal analysis. Frequency analysis revealed two asymptotes in the Bode plot of the data; and the intersection of the asymptotes (corner frequency) was higher for the B+/- strain (8.73±1.10Hz vs 16.56±1.26Hz, P<0.05), consistent with a higher overall cross-bridge cycling rate in heterozygous NMIIB KO (B+/-) vs WT mice. These results demonstrate that because of their long duty cycle, NMIIB cross-bridges act as an internal load on smooth muscle myosin to decrease the overall cross-bridge cycling rate and muscle Vmax during force maintenance. © 2010 Federation of European Biochemical Societies.