Stability of SARS-CoV-2 antibody in serum under various usage and storage conditions
Journal of Infection in Developing Countries, cilt.18, sa.9, 2024 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 18 Sayı: 9
- Basım Tarihi: 2024
- Doi Numarası: 10.3855/jidc.18919
- Dergi Adı: Journal of Infection in Developing Countries
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, MEDLINE, Veterinary Science Database, Directory of Open Access Journals
- Anahtar Kelimeler: freeze-thaw, SARS-CoV-2 IgG, serum, stability
- Maltepe Üniversitesi Adresli: Evet
Özet
Introduction: We investigated the effect of two preanalytical variables, temperature change and freezing-thawing of serum samples, on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) IgG levels. Methodology: Serum samples were collected from patients who had coronavirus disease 2019 (COVID-19) prior to vaccination. Six serum samples were included, two each with high positivity (HP), low positivity (LP), and a level of close-to-detection limit (CDL) for SARS-CoV-2 IgG. Each of these six samples was divided into three tubes and placed in refrigerators at 4-8°C, -20°C, and -70°C; removed from the storage temperature once per day for 20 consecutive days; and assayed for SARS-CoV-2 IgG level. Results: The coefficient of variation of all the remaining serum samples were within 95% except for CDL-1 serum at -70°C, HP-2 serum at 4-8°C, HP-2 serum at -20°C, and HP-2 serum at -70°C. The levels increased significantly when the temperature in the samples with CDL was reduced. The values in samples with LP at -20°C and -70°C were significantly higher than those at 4-8°C. In the case of samples with HP, the values of samples at -20°C were higher than those in samples at 4-8°C. There was no positive-negative change during any of the freeze-thaw cycles. Conclusions: Antibody value in the samples at 4-8°C remained stable throughout the 20 freeze-thaw cycles. The antibody value of the samples at -20°C and -70°C tended to elevate.