Galactose-induced aging model in rat testicular tissue
Journal of the College of Physicians and Surgeons Pakistan, cilt.28, sa.7, ss.501-504, 2018 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 28 Sayı: 7
- Basım Tarihi: 2018
- Doi Numarası: 10.29271/jcpsp.2018.07.501
- Dergi Adı: Journal of the College of Physicians and Surgeons Pakistan
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.501-504
- Anahtar Kelimeler: Galactose, Mimetic aging, Natural aging, Oxidative stress, Redox homeostasis, Testis
- Maltepe Üniversitesi Adresli: Evet
Özet
Objective: To examine whether the D-galactose induced aging model is an appropriate model for further aging research. Study Design: Experimental study. Place and Duration of Study: Aziz Sancar Institute of Experimental Medicine, Istanbul University, Turkey, June 2015- June 2017. Methodology: The study comprises 3 groups of rats. Group I is young control (YC) 5-month-old rats. Group II is 5-monthold rats, which were mimetically aged (MA) for 6 weeks via intraperitoneal D-galactose (60 mg/kg body weight/day, 0.5 mL) administration. Group III is naturally aged (NA) 24-month-old rats. Group I and III received intraperitoneal saline (0.9% 0.5 mL) for 6 weeks as vehicle. Group I and Group II received injections at 21 weeks age and Group III rats 6 weeks before 24 months age. Tissues were harvested when rats became 6.5-month-old (Group I and Group II) and 24-month-old (Group III). Quantitative biochemical analyses of proteins, lipids, DNA biomarkers and Cu, Zn-SOD were conducted. Statistical analysis of the data was conducted using ANOVA, followed by post-hoc Bonferroni test. Results: Higher magnitude of oxidative damage and diminished antioxidant defence capacity were found in both mimetically aged and naturally aged testicular tissues. It is observed that D-galactose aging model group shares significant similarities in terms of impaired redox homeostasis with the naturally aged rats. Conclusion: D-galactose induced testicular aging model successfully mimics aging process. Therefore, D-galactose induced aging model may be used as an accelerated aging model to study the age related alterations and interventions.