A conserved role for COMA/CENP-H/I/N kinetochore proteins in the spindle checkpoint
Genes and Development, cilt.26, sa.6, ss.542-547, 2012 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 26 Sayı: 6
- Basım Tarihi: 2012
- Doi Numarası: 10.1101/gad.184184.111
- Dergi Adı: Genes and Development
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.542-547
- Anahtar Kelimeler: Kinetochore, Mitosis, Spindle assembly checkpoint
- Maltepe Üniversitesi Adresli: Hayır
Özet
The COMA/CENP-H/I kinetochore complex regulates microtubule dynamics at kinetochores. The complex is also required to generate spindle checkpoint signals in both yeast and human cells under conditions where Aurora B activity is compromised. Our data explain why mammalian cells treated with Aurora inhibitors still have a functional spindle assembly checkpoint (SAC), since the checkpoint signals through CENP-H/I/N. The SAC effect from depleting the CENP-H/I/N complex cannot be explained by a weakened SAC signal, and the complex has no role in the SAC response to paclitaxel. We propose a model to explain the differential response of human cells to nocodazole and paclitaxel. © 2012 by Cold Spring Harbor Laboratory Press.